Table 1. Bacterial strains constructed in this study.
| Bacterial strain | Genotype or insert | Transformation | Antibiotic selectiona | PCR | Reference or source |
|---|---|---|---|---|---|
| Escherichia coli | |||||
| JA221 | F– hsdR hsdM+ trp leu lacY recA1 | (31) | |||
| Bacillus subtilis | |||||
| RM125 | leuB8 arg-15 ΔSPβ | (23) | |||
| BEST7003b | RM125 plus proB::pBRTc Pr-neo | Nm, Tc | This study | ||
| BEST6250 | proB::pKD[2/1]A | pKD[2/1]A × BEST7003 | Spc, Em | This study | |
| BEST6257 | proB::pBR[120] | pKANED × BEST6250 | Nm, Em | P1, P2, P3 | This study |
| BEST6265 | proB::pBR[110] | pCISP320 × BEST6257 | Spc, Em | P1, P2, P3 | This study |
| BEST6269 | proB::pBR[110] | pCISP320B × BEST6265 | Nm, Em | P1, P2, P3 | This study |
| BEST6283 | proB::pBR[86] | pKDC 302 × BEST6269 | Cm, Spc | P1, P2 | This study |
| BEST6287 | proB::pBR[34] | pKDS103 × BEST6269 | Cm, Spc | P1 | This study |
| BEST6280 | proB::pBR[16.5] | pKDC202 × BEST6269 | Cm, Spc | P1 | This study |
| BEST6273 | proB::pBR[11.2] | pKane301 × BEST6269 | Em, Spc | P1 | This study |
| BEST6412 | proB::pBR[34], pGETS1006 | pGETS109 × BEST6287 | Cm, Spc, Tc | P1 | This study |
| BEST6410 | proB::pBR[16.5], pGETS1004 | pGETS109 × BEST6280 | Cm, Spc, Tc | P1 | This study |
| BEST6426 | proB::pBR[11.2], pGETS1011 | pGETS103 × BEST6273 | Em, Spc, Tc | P1 | This study |
aNm, neomycin resistance; Spc, spectinomycin resistance; Cm, chloramphenicol resistance; Em, erythromycin resistance; Tc, tetracycline resistance.
bThe Pr-neo gene is inserted between NotI sites of yvfC and yveP.