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. 2003 Sep 15;31(18):e113. doi: 10.1093/nar/gng115

Figure 2.

Figure 2

Structure of pPCR102-2 and its derivatives encoding different modules of the proliferation-inducing cDNA. In pPCR102-2, the novel proliferation-inducing cDNA is flanked by viral sequences and contains an ORF. In order to narrow down the proliferation-inducing ORF, we constructed pND-A8 containing 5′ deletions of viral sequences, pND-A2 harboring a deletion of the ATG start codon of ORF and pPI1-His encoding full-length PI1 ORF fused in-frame to a histidine tag. pPCR102-2 derivatives were produced by deleting sequences either upstream or including the putative start ATG of the ORF. All constructs were cloned under control of PhCMV.