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Expression of the PI1 protein. Escherichia coli transgenic for pDC106 harboring PI1-His (see Fig. 2) under control of the IPTG-inducible Plac promoter was grown for 4 h in the presence (+) or absence (–) of IPTG. Cell lysates were resolved on SDS–PAGE and analyzed for PI1-His production by His-tag-targeted immunodetection.
