Fig. 4. Surface plasmon resonance confirms FYB and FYN SH3 domain binding to TRAKGDYASYYQG peptide. Binding of the TRAKGDYASYYQG peptide to immobilized FYB SH3 domain (upper panel), FYN SH3 domain (middle panel) and GRB2 N-terminal SH3 domain (lower panel). Surface plasmon resonance was conducted as described in Materials and methods. Peptide TTGVFVKMPPTE was used as an irrelevant control. Peptides were injected at 35, 70, 175 or 350 µM. Binding was measured in resonance units (RU), where 1 RU corresponds to the binding of 1 ng/mm² of flow cell surface.