Fig. 8. (A) Evi-1 inhibits JNK in U937 cells. pcDNA3-Evi-1 was introduced into U937 cells and cells were selected for G418 resistance. The mock- and the Evi-1-transfected cells were either left untreated or treated with 10 nM TNF-α for 1 h as indicated. Then endogenous JNK1 was immunoprecipitated with anti-JNK1, and the kinase assay was performed (top). Expression of endogenous JNK1 along with Evi-1 was monitored as indicated (middle and bottom). (B) Blocking of TNF-α-induced apoptosis by Evi-1 in U937 cells. The mock- (lanes 1–3) and the Evi-1-transfected U937 cells (lanes 4–6) were treated with the indicated doses of TNF-α for 24 h. Cellular DNA was extracted and analyzed by agarose gel electrophoresis.