Fig. 4. Developmental expression of PfEMP3, PfEMP1 and KAHRP in 3D7k⁺ and EMPΔ1. Synchronized ring stages of each parasite line were sampled every 8 h and Triton X-100-insoluble/SDS-soluble fractions analysed by SDS–PAGE. The same extract from 3D7k⁺ was probed with (A) anti-PfEMP3, (B) anti-ATS, (C) anti-KAHRP and (D) anti-Hsp70 antibodies. Extracts from EMPΔ1 were probed with (E) anti-PfEMP3, (F) anti-ATS, (G) anti-KAHRP and (H) anti-Hsp70 antibodies.