. 2000 Jun 15;19(12):2856–2868. doi: 10.1093/emboj/19.12.2856

Table I. Influence of specific phages on cell-to-cell movement of endogenous and viral movement proteins when coinjected into mesophyll cells of fully expanded leaves of Nicotiana benthamiana.

Injected probe	Coinjected agent	Injections	Movement (%)
Lucifer yellow CH	–	45	44 (98)^a
11 kDa FITC-dextran	–	55	3 (5)^b
KN1	F-dextran^c	48	39 (81)^a
CMV-MP	F-dextran	18	15 (83)^a
W2 proteins	F-dextran	21	19 (90)^a
KN1-FITC	–	33	29 (88)^a
ph-KN1pep^d	F-dextran	37	1 (3)^b
ph-KN1pep^e	KN1 + F-dextran	39	9 (23)^f
ph-KN1pep	W2 proteins + F-dextran	30	9 (30)^f
ph-KN1pep	KN1-FITC	57	31 (54)^g
ph-KN1pep	CMV-MP + F-dextran	30	17 (57)^h
ph-CMVpep2^d	F-dextran	15	2 (13)^b
ph-CMVpep2	KN1 + F-dextran	30	6 (20)^f
ph-CMVpep2	W2 proteins + F-dextran	25	3 (12)^f
ph-CMVpep2	KN1-FITC	26	20 (77)^g
ph-CMVpep2	CMV-MP + F-dextran	30	6 (20)^f
ph-CMVpep3^d	F-dextran	22	4 (18)^b
ph-CMVpep3	KN1 + F-dextran	35	19 (54)^h
ph-CMVpep3	W2 proteins + F-dextran	23	18 (78)^a
ph-CMVpep3	KN1-FITC	31	21 (68)^a
ph-CMVpep3	CMV-MP + F-dextran	35	13 (37)^f
ph-KN1pep	KN1 + CMV-MP + F-dextran	14	4 (29)^f
ph-CMVpep3	KN1 + CMV-MP + F-dextran	13	5 (38)^f
ph-empty	KN1 + CMV-MP + F-dextran	23	18 (78)^a
ph-empty^d	F-dextran	20	3 (15)^b
ph-empty	KN1 + F-dextran	41	27 (65)^h
ph-empty	KN1-FITC	15	11 (73)^a
ph-empty	W2 proteins + F-dextran	23	18 (78)^a
ph-empty	CMV-MP + F-dextran	28	22 (79)^a

^aFluorescent signal associated with the reporter probe moved through >10–15 surrounding mesophyll cells.

^bWhen movement was detected, the fluorescent signal was generally limited to adjacent cells.

^cCoinjection of 11 kDa FITC-labeled dextran (F-dextran) allowed monitoring of plasmodesmal SEL increase and cell-to-cell movement of unlabeled KN1, W2 fraction proteins or CMV-MP.

^dConcentration of various phage particles used in microinjection experiments was 1 µg/µl.

^eRatio of phage particles to KN1, W2 fraction proteins or CMV-MP was ∼1:4 (w/w).

^fWhen the fluorescent signal moved it did so slowly and was mostly restricted to the adjacent cell(s).

^gRate of KN1-FITC movement was much slower relative to the controls.

^hF-dextran signal was detected in 5–10 surrounding mesophyll cells.