Table II. Antagonistic effects of synthetic oligopeptides on the capacity of KN1 and CMV-MP to interact with mesophyll plasmodesmata located within fully expanded leaves of Nicotiana benthamiana.
Injected probe | Coinjected agent | Injections | Movement (%) |
---|---|---|---|
Lucifer yellow CH | – | 18 | 17 (94)a |
11 kDa FITC-dextran | – | 40 | 7 (18)b |
KN1 | F-dextranc | 13 | 11 (85)a |
W2 proteins | F-dextran | 10 | 8 (80)a |
CMV-MP | F-dextran | 10 | 8 (80)a |
KN1-FITC | – | 22 | 18 (82)a |
KN1pepsynth | F-dextran | 37 | 13 (35)b |
KN1pepsynthd | KN1 + F-dextran | 38 | 7 (18)b |
KN1pepsynth | KN1-FITC | 39 | 23 (59)e |
CMVpep3synth | F-dextran | 17 | 3 (18)b |
CMVpep3synthd | CMV-MP + F-dextran | 14 | 7 (50)e |
CMVpep3synth | KN1 + F-dextran | 19 | 12 (63)f |
CMVpep3synth | KN1-FITC | 18 | 15 (83)a |
KN1-Npepsynth | F-dextran | 20 | 5 (25)b |
KN1-Npepsynthd | KN1 + F-dextran | 20 | 3 (15)b |
KN1-Npepsynth | W2 proteins + F-dextran | 22 | 3 (14)b |
KN1-Npepsynth | KN1-FITC | 20 | 5 (25)b |
KN1-Npepsynth | CMV-MP + F-dextran | 22 | 18 (70)a |
a–cAs in Table I.
dRatio of synthetic peptide to protein was 3:1 (w/w).
eWhen the fluorescent signal moved it did so slowly and was mostly restricted to the adjacent cell(s).
fF-dextran signal was detected in 5–10 surrounding mesophyll cells.