Table III. Synthetic KN1 oligopeptides block KN1-mediated cell-to-cell transport of KN1–sense RNA.

Injected probea	Coinjected agent	Injections	Movement (%)
Lucifer yellow CH	–	24	24 (100)b
11 kDa F-dextran	–	23	3 (13)b
KN1	F-dextranc	25	21 (84)b
KN1-FITC	–	10	9 (90)b
KN1–sense RNA-CFd	KN1	21	17 (81)b
KN1–sense RNAe	KN1-FITC	6	5 (83)b
KN1–sense RNA	KN1-FITC + ph-empty	11	8 (73)b
KN1–sense RNA	KN1-FITC + ph-KN1pep	9	2 (22)f
KN1–sense RNA-CF	KN1 + CMVpep3synth	10	7 (70)b
KN1–sense RNA-CF	KN1 + KN1pepsynth	21	2 (10)f
KN1–sense RNA-CF	KN1 + KN1-Npepsynth	18	3 (16)f

^aAll probes were injected into mesophyll cells located in fully expanded leaves of N.benthamiana.

^bFluorescent signal associated with the reporter probe moved through >10–15 surrounding mesophyll cells.

^cCoinjection of 11 kDa F-dextran allowed monitoring of plasmodesmal SEL increase and cell-to-cell movement of unlabeled KN1.

^dConcentrations of KN1–sense RNA (unlabeled and covalently labeled with CF), KN1/KN1-FITC and KN1pep_synth/KN1-Npep_synth/CMVpep_synth used in these experiments were 10 ng/µl, 2.5 µg/µl and 1.0 µg/µl, respectively.

^eConcentrations of KN1–sense RNA, KN1/KN1-FITC and ph-KN1pep/ph-empty used in these experiments were 10 ng/µl, 2.5 µg/µl and 1.0 µg/µl, respectively.

^fWhen movement was detected, the fluorescent signal was generally limited to adjacent cells.