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. 1997 Apr 1;94(7):3145–3150. doi: 10.1073/pnas.94.7.3145

Figure 1.

Figure 1

Isolation of putative global negative regulators. (a) Genetic selection for suppressors of the temperature-sensitive (ts) SRB4 mutant RNA polymerase II holoenzyme. (b) Sequence of NCB1 (ORF YER159c on chromosome V, GenBank accession no. U18917U18917). The suppressing allele, ncb1-1, was isolated by gap-repair techniques and sequenced. The suppressing mutation, a single base pair deletion at nucleotide 340, is noted in boldface type. The deletion results in a frameshift causing a translational stop at nucleotides 347–349, also noted in boldface type. Underlined regions indicate homology to α-helices in the histone H2A histone fold (42–44). (c) Sequence of NCB2 (ORF D9509.16 on chromosome IV, GenBank accession no. U32274U32274) with the first and last nucleotide of the intron sequence noted in boldface type. Underlined regions indicate homology to α-helices in the histone H2B histone fold (42–46).