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Yeast NC2 binds to TBP and can be purified as a two-subunit complex. (a) Western blot analyses of TBP column onput and eluates with antibodies against yNC2α and yNC2β. Bound proteins were eluted with 2 M KCl. (b) Silver-stained SDS/polyacrylamide gel and Western blot analyses of fractions from the final step of the purification (DEAE 5PW).
