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. 1997 Apr 1;94(7):3145–3150. doi: 10.1073/pnas.94.7.3145

Figure 3.

Figure 3

Highly purified yeast NC2 inhibits transcription by RNA polymerase II holoenzyme in vitro. (a) Analysis of NC2 Mono Q fractions by SDS/PAGE and silver staining. (b) Western blot analyses of Mono Q fractions. (c) Influence of Mono Q fractions on in vitro transcription by RNA polymerase II holoenzyme. (d) Inhibition of in vitro transcription by RNA polymerase II holoenzyme with increasing amounts of purified yeast NC2. Assuming a molecular weight of 64,000 for NC2, 0.5 pmol was required for 50% inhibition of an equimolar amount of RNA polymerase II holoenzyme (estimated molecular weight of 2 million) and TBP.