Fig. 1. 4E-T cDNA. (A) Nucleotide and amino acid sequence of human 4E-T. The first methionine is circled. The stop codon and polyadenylation signal are underlined and the eIF4E-binding site is boxed. The nuclear localization signal and nuclear export signals are highlighted with gray and black boxes, respectively. The 4E-T sequence has been deposited in the DDBJ/EMBL/GenBank database (accession No. AF240775). (B) Northern blotting analysis of 4E-T. HeLa poly(A)⁺ RNA (5 µg) was probed with a 4E-T 5′ end probe as described in Materials and methods.