Fig. 3. Incorporation of [³H]DHS into sphingolipids and DHS-1P. The lcb1-100 (RH3809) or triple mutants (RH3859, lcb1/3/ ysr3 and RH4355, lcb1/4/5) were labeled with [³H]DHS at 37°C. Lipids were analyzed by TLC using solvent system II. Images of portions of individual lanes are shown and were scanned to quantify the incorporation. The incorporation of [³H]DHS into DHS-1P or sphingolipids in the lcb1-100 mutant was set to 100%. The positions of PI, IPC, DHS-1P, M(IP)₂C, as well as two unknown products, Y and Z, are marked.