Fig. 8. Actin staining in wild-type and mutant cells. RH1800 (wt) or RH3809 (lcb1) cells were incubated at 24 or at 37°C in the presence or absence of DHS or PHS, then fixed, and filamentous actin was visualized using TRITC–phalloidin (left panels) or Nomarski optics (right panels).