Fig. 1. Loss of PP2A function suppresses the lcb1-100 mutation for endocytosis. (A) Wild-type RH1800 (wt) and single mutant strains RH3745 (cdc55), RH4137 (pph-ts) and RH4183 (sit4-ts) were assayed for α-factor internalization at 37°C. All mutants showed almost wild-type internalization kinetics. (B) The RH3809 strain (lcb1-100) and double mutant strains, RH3807 (lcb1 cdc55), RH4191 (lcb1 pph-ts) and RH4195 (lcb1 sit4-ts) were assayed for α-factor internalization at 37°C.