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. 2000 Jun 15;19(12):2834–2844. doi: 10.1093/emboj/19.12.2834

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Copyright © 2000 European Molecular Biology Organization

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graphic file with name cdd307f5.jpg — Fig. 5. (A) lcb1-100 and yck-ts mutations have a synthetic effect on endocytosis. The single mutant lcb1-100 (RH3802) and yck-ts (RH4336) cells were assayed for α-factor uptake at both 24°C (open symbols) and 37°C (closed symbols) and compared with the double mutant lcb1 yck-ts (RH4337) cells. (B) Radiolabeled α-factor uptake assays were performed at 24°C (open symbols) or 37°C (closed symbols) on wild-type (RH448), pkc1-ts (RH4325), lcb1-100 (RH3802) and lcb1 pkc1-ts (RH4329) strains. (C) Internalization assays were performed at 24°C (open symbols) and 37°C (closed symbols) on pkc1-ts (RH4325), yck-ts (RH4742) and yck-ts pkc1-ts (RH4598) mutants.