Fig. 7. Loss of function of P2A or kinase overexpression suppresses the lcb1-100 actin organization defect. Logarithmic cultures of wild-type (WT, RH448), lcb1 (RH3802), lcb1 cdc55 (RH3807), lcb1 pph-ts (RH4191) and lcb1-100 (RH3802) cells with high copy number plasmid containing either YCK2 or PKC1 genes (lcb1+YCK2 or lcb1+PKC1) and end7-1 (act1, RH2069) cells with or without the PKC1 plasmid (end7+PKC1) were grown at 24°C, shifted to 37°C for 2 h, fixed, stained with TRITC–phalloidin and observed by fluorescence (actin) and Nomarski (DIC) microscopy.