Figure 3.
Immunoprecipitation of β′2 subunit precursor from mutant Jurkat-β2.7 cells that lack leukocyte integrin α subunits. Mutant Jurkat-β2.7 T lymphoblastoid cells were pulsed with [35S]cysteine for 1 h and chased with unlabeled cysteine for 12 h. Lysates were immunoprecipitated with X63 myeloma IgG1 control (lane 1), mouse anti-human LFA-1 β subunit (lanes 2–17), and mouse anti-human LFA-1 α subunit (lane 18) mAbs and subjected to reducing SDS/7.5% PAGE. The position of the β′2 precursor is indicated.