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. 1997 Apr 1;94(7):3156–3161. doi: 10.1073/pnas.94.7.3156

Figure 4.

Figure 4

Immunoprecipitation of pulse-labeled and pulse-chased murine LFA-1. The murine T cell line, EL-4 was pulsed for 1 h with [35S]cysteine, and half of the cells were chased for 16 h with unlabeled cysteine. Cell lysates were immunoprecipitated with control X63 myeloma IgG1, rat anti-mouse LFA-1 α subunit mAb M7/14, or rat anti-mouse β2 mAb C71/16. The precipitates were subjected to reducing SDS/7.5% PAGE. The positions of the mature αL, precursor α′L, mature β2, and precursor β′2 subunits are indicated.