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Immunoprecipitation of mature LFA-1. JY lymphoblastoid cells were labeled for 1 h with [35S]cysteine and chased for 16 h with unlabeled cysteine. Lysates were immunoprecipitated with mouse anti-human LFA-1 antibodies or X63 myeloma IgG as control and subjected to reducing SDS/7.5% PAGE and fluorography. The positions of molecular weight standards, myosin (200 kDa), β-galactosidase (116 kDa), phosphorylase b (97 kDa), and serum albumin (66 kDa), are marked.
