Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Immunoprecipitation of unassociated α′L and β′2 subunit precursors. JY lymphoblastoid cells were pulsed with [35S]cysteine for 1 h. Cell lysates were immunoprecipitated with mouse anti-human LFA-1 mAb or X63 myeloma IgG as control, and subjected to reducing SDS/7.5% PAGE and fluorography. As a control, mature LFA-1 was precipitated with TS1/22 from lysates of JY cells that were pulsed for 1 h and chased for 16 h (lane 14).
