Figure 5.

Immunoprecipitation of unassociated α′_L precursor from a leukocyte adhesion deficiency patient cell line. A B lymphoblastoid cell line established from a deficient patient was pulsed for 1 h with [³⁵S]cysteine and chased for 12 h with unlabeled cysteine. Cell lysates were immunoprecipitated with LFA-1 mAb or X63 myeloma IgG as control and subjected to reducing SDS/7.5% PAGE. Lanes 1–11 and 12–14 are from two separate immunoprecipitation experiments. As a control, lysates from pulse-labeled and chased JY cells were used in lane 1.