Table 1.
Adhesive interactions of cells with immobilized antibodies
| Antigen | Antibody
|
Antigen expression
|
Adhesion† | ||
|---|---|---|---|---|---|
| Name | Class | Cell line | MESF* | ||
| CD15s | CSLEX-1 | IgM | JFT5 | 1188 | R |
| HL-60 | 6433 | R | |||
| JPuro | 0 | N | |||
| CD15 | PM-81 | IgM | PMN | 2133 | R |
| JFT5 | 3951 | R | |||
| HL-60 | 7079 | R | |||
| JPuro | 270 | R | |||
| CD15 | IGR-15B6 | IgM | JFT5 | 2530 | R |
| HL-60 | 8828 | R | |||
| JPuro | 343 | N | |||
| CD15 | mAb80 | IgM | JFT5 | 1649 | T/R |
| HL-60 | 6187 | T/R | |||
| JPuro | 29 | N | |||
| CD15 | 2TAP146 | IgM | JFT5 | 459 | T/R |
| HL-60 | 7350 | T/R | |||
| JPuro | 2 | N | |||
| Lewisa,b | HEA164 | IgM | RD3/5 | 2380 | r |
| Hela | 7 | N | |||
| CD108 | MEM-121 | IgM | JY | 139 | F/r |
| CD48 | MEM-124 | IgM | JY | 2514 | R |
| CD59 | MEM-125 | IgM | Hela | 7495 | r |
| HL-60 | 554 | R | |||
| CD59 | C2G4 | IgM | Hela | 6526 | F/r |
| HL-60 | 620 | F/r | |||
| CD55 | MEM-118 | IgM | JY | 293 | F |
| HL-60 | 109 | F | |||
| JPuro | 172 | F | |||
| CD45 | NK45 | IgM | HL-60 | 881 | F |
| JPuro | 1678 | F | |||
| CD50 | CBR-IC3/1 | IgG | HL-60 | 663 | F |
| JPuro | 581 | F | |||
| CD50 | CBR-IC3/2 | IgG | HL-60 | 652 | F |
| JPuro | 644 | F | |||
| CD50 | CBR-IC3/6 | IgM | HL-60 | 391 | F |
| JPuro | 362 | F | |||
| CD11a | CBR LFA-1/10 | IgM | KG1a | 777 | F |
| HL-60 | 369 | F | |||
| CD11a | CBR LFA-1/9 | IgM | KG1a | 1475 | F |
| HL-60 | 554 | F/r | |||
| JPuro | 128 | F/r | |||
| CD11a | CBR LFA-1/1 | IgM | KG1a | 1150 | F |
| HL-60 | 458 | F | |||
| JPuro | 117 | F | |||
| CD62L | DREG56 | IgG | JPuro | 309 | F |
| HL-60 | 18 | N | |||
| CD62L | DREG200 | IgG | JPuro | 142 | F |
| CD62L | LAM1-3 | IgG | JPuro | 419 | F/r |
| CD43 | L10 | IgG | KG1a | 1056 | F |
| HL-60 | 1944 | F | |||
| JPuro | 582 | F | |||
| CD43 | BRA7G | IgM | KG1a | 1144 | F |
Mean immunofluorescence intensity was standardized with fluorescein isothiocyanate-conjugated beads from Flow Cytometry Standards (San Juan, Puerto Rico) to calculate molecules of equivalent soluble fluorochrome ×10−3 (MESF).
Interactions were classified based on the motion of cells tethered to the substrate in shear flow and subjected to subsequent increases in shear. R, rolling, at least 50% of attached cells would eventually roll for more than five cell diameters with a velocity greater than 1 μm s−1 before detachment; r, slow rolling, at least 50% of cells would roll for more than five cell diameters with a velocity less than 1 μm sec−1 before detachment; T, transient tethering, cells would be tethered to the substrate for a certain time and then detached in the flow, and the detached cells might be tethered again downstream; F, firm adhesion, attached cells stuck to the substrate until detachment by a higher shear; the cells moved less than two cell diameters before reaching the hydrodynamic velocity, and once detached the cells were rarely attached again; F/r, cells would slowly roll for two to five cell diameters at high shear before detachment; N, no adhesion, cells did not attach to the substrate.