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. Author manuscript; available in PMC: 2008 Oct 15.
Published in final edited form as: Bioorg Med Chem. 2007 Jul 25;15(20):6565–6573. doi: 10.1016/j.bmc.2007.07.001

Table 2.

The 2:1 complex formation of two DQ2 molecules binding to one peptide

Peptides 2:1 complex formation at pH 5 (%)b
9 <10
10 18
8 22
11 21
12 22
13 26
14 25
15 26
b

Recombinant DQ2 (4.7 μM) was mixed with fluorescein-conjugated peptide (0.185 μM) at 37°C for 45 h at pH 5.5. The formation of 2:1 DQ2-peptide complex with two DQ2 molecules binding to one peptide was reported as the percentage among total DQ2-peptide complex, i.e. [2:1 complex] / ([2:1 complex] + [1:1 complex]) ×100%.