Figure 5.

Signal transduction experiments of IL-10 induction by Nef using several inhibitors. PBMCs were pretreated separately for 2 h with medium or inhibitor alone or were additionally stimulated with 500 ng/ml yeast-derived Nef alone or with 1.4 mM calcium chloride for 40 h. IL-10 production in the supernatants of PBMC from two different individuals was detected by ELISA. In Exp. 1, inhibitors used were 50 μM W-7, 1.5 nM staurosporine, and 100 nM H-89. In Exp. 2, inhibitors used were 50 μM W-7, 1.5 nM staurosporine, 100 nM H-89, 100 nM calphostin C, and 1 mM EGTA.