Fig. 2.

Action potential firing of Purkinje cells in awake WT and RNB transgenic mice. (a) Spontaneous activities of Purkinje cells consisted of simple spikes and complex spikes (red asterisks) in WT, DOX-untreated, and DOX-withdrawn RNB mice, but no simple spikes were evoked in DOX-treated RNB mice. (b) Traces of simple spikes shown in a were superimposed. (c) Interspike interval histogram of simple spikes shown in b. (d) Traces of five complex spikes were superimposed. (e and f) Mean ± SEM of firing rates of simple spikes (e) and complex spikes (f) are shown with columns and bars, respectively. ***, P < 0.001 (comparison between DOX-treated RNB mice and all five other animals by the Scheffé test). (g) The extracellular recording site (red) was confirmed at the Purkinje cell layer by fluorescent Nissl staining. ML, molecular layer; PL, Purkinje cell layer; GL, granule cell layer.