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Western blot analysis of proteins from pYEXTHS-BN-Tpi-PGFAD transformed yeast grown in the presence of different CuSO4 concentrations. Proteins were extracted from cell lysates and separated by SDS/PAGE. Fifteen micrograms of protein were loaded in each lane. The hexahistidine-tagged protein was detected by immunoblotting with an anti-His6 antibody. The size of the molecular mass marker is indicated on the right.
