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. 2007 Oct 9;104(42):16657–16662. doi: 10.1073/pnas.0707936104

Fig. 4.

Fig. 4.

β-arrestin2-GFP translocation to the β2AR-V2R and receptor internalization stimulated by carvedilol. HEK-293 cells transiently expressing the β2AR-V2R chimera were stimulated for 2 min with either isoproterenol (Iso), carvedilol (Carv), or propranolol (Prop). (A) β-arrestin2-GFP translocation to the β2AR-V2R was then monitored by confocal microscopy. Images are representative of six independent experiments. (B) HEK-293 cells stably expressing β2AR were stimulated with 10 μM of ligand for 30 min and assayed for internalization by fluorescence-activated cell sorting. Data represent mean ± SE of five independent experiments done in duplicate. *, P < 0.05 vs. NS, ***, P < 0.0001 vs. NS.