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. 2007 Oct 10;104(42):16558–16563. doi: 10.1073/pnas.0702581104

Fig. 4.

Fig. 4.

Ovotestis formation in XY SF1-Cre/+;Fgfr2flox/flox gonads at 15.5 dpc. (A–C) Stereomicroscopy images of urogenital systems of controls and XY SF1-Cre/+;Fgfr2flox/flox mutant. In addition to gonadal defects, the adrenal glad is severely reduced or absent in these mutants. Ad, adrenal; Ov, ovary; Ts, testis; *, mutant adrenal and gonad. (D–H) Histology of wild-type XY and SF1-Cre/+;Fgfr2flox/flox XY gonads at 15.5 dpc. (E–H) Higher magnification views show testis cords in the wild-type XY gonad (F), disrupted cords from the central region in the mutant XY gonad (G), and ovarian region (H) located in the anterior of the mutant gonad (bracket in E). Arrow indicates the nucleus of a germ cell in meiosis. (I–K) Confocal scanning microscopy of sections of 15.5 dpc gonads immunostained with SOX9 (green) and SCP3 (red). (I and J) SCP3-positive germ cells are characteristic of wild-type XX gonads, but not XY gonads at this stage, whereas, SOX9 shows the reverse pattern. (K′–K‴) The ovotestis contains a region of SCP3-positive cells in the anterior domain (arrowhead in K′), a region where both markers are present (dashed line in K″), and a separated cluster of SOX9-positive cells where SCP3 is absent (arrow in K‴).