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. 2007 Oct 10;104(42):16486–16491. doi: 10.1073/pnas.0706847104

Table 1.

Purification of DdPPK1

Sample Specific activity, Units/mg × 104 Total activity*, units × 105 Purification, fold Recovery, %
WT cell lysate 0.1
Overproducer 1.0 17.7 10
Heparin 15 6.7 150 38
FT of DEAE 17 5.4 170 30
Mono Q column 48 1.5 480 8.5

*One unit of enzyme is defined as the amount incorporating 1 pmol of phosphate into poly P per min at 37°C.