Fig. 1.

Overview of the Oct4-sorted series. (A) FACS in an ESC to EB differentiation time course followed by expression profiling. The cells were separated by FACS into undifferentiated (GFP+) and differentiated (GFP−) subpopulations, as indicated by green and white solid circles, respectively. Microarray expression profiling was performed on the sorted subpopulations of cells, and we used the resulting data as the basis for identifying Oct4-sorted± genes. (B) Expression levels of selected marker genes in the Oct4-high and -low samples as measured by the percentage of maximal expression. The error bars indicate the standard errors of the expression levels. (C) Overlaps between the gene sets in the Oct4-sorted, Oct4-RNAi, and RA-induction series. The numbers in the Venn diagrams represent the counts of the contiguous regions (gene sets) where they appear. The P values of the overlaps are calculated by the hypergeometric distribution (SI Text).