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. 2007 Oct 10;104(42):16708–16713. doi: 10.1073/pnas.0708136104

Fig. 1.

Fig. 1.

Expression of SERT and 5-HT receptors in mammary epithelial cells. (A) RT-PCR and Western blot for SERT in human brain and MCF10A cell extracts. (B) (i) RT-PCR for 5-HT receptors in positive control brain and MCF10A cell extracts, along with the housekeeping gene (GAPDH). Selective primers distinguished the expression of three known human splice variants of HTR7. (ii) Western blot for 5-HT7 in human and mouse mammary cells, with brain from each species as a positive control. (C) cAMP accumulation in MCF10A cells in response to 5-HT. Averages (±SEM) are of cells from four dishes. The positive control of cells incubated with 2 μM forskolin was measured to be 12.6 ± 0.6 pmol/ml. The result is representative of three experiments.