Fig. 5.
Mechanism of tight junction regulation in MCF10A cells. (A) Basal polarity of 5-HT7 receptor functionality in MCF10A membranes. Data are expressed as the percentage change of experimental vs. control values (±SEM) measured 48 after drug addition. Bars are labeled according to the drugs applied: HIAA, 2.5 mM 5-hydroxyindole acetic acid; 5-HT, 2.5 mM serotonin. Drug treatment was given on both sides (filled bar), apical side only (shaded bar), and basal side only (open bar). (B) Western blot for tight junction proteins with GAPDH as loading control on MCF10A transwell lysates in control (untreated) and 0.75 mM 5-HT (treated) conditions.