GCL activity and GCLC protein expression in patients and controls under untreated and t-BHQ-treated conditions. (A) GCL activity of 25 patients and 25 controls, expressed as nanomoles of GSH per minute per milligram of protein. Each box describes 25%, 75%, and median values. The whisker bars show values in the 1.5 box lengths range. The open circle depicts an outlier value. (B) The plot shows GCLC protein levels of 26 patients and 26 controls, determined as α-tubulin corrected arbitrary units. **, P < 0.01; ***, P < 0.001 vs. the respective controls were calculated by using ANOVA test (two-tailed). (C) Representative Western blots of GCLC, GCLM, and α-tubulin of two controls and two patients under untreated and t-BHQ-treated conditions.