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. 2007 Sep 19;104(42):16564–16569. doi: 10.1073/pnas.0707537104

Fig. 1.

Fig. 1.

Mos requires Emi2 to induce M phase arrest. (A) Cycling extracts were incubated at room temperature for 45 min and were then supplemented with MBP or MBP-Mos. Aliquots were withdrawn at indicated times and immunoblotted for phosphoMAPK (pMAPK), cyclin B2, cyclin A, and phosphoCdc2 (pCdc2). (B) Two sets of cycling extracts in the presence of control or Emi2 antisense morpholinos were incubated at room temperature for 45 min. Mos protein was added to one set of the extracts. Samples were taken at indicated times. Samples taken from extracts without Mos were used to immunoblot for Emi2, and samples taken from extracts with Mos were used to immunoblot for pMAPK and cyclin B2. The asterisk marks a nonspecific band. (C) Cycling extracts were incubated at room temperature in the presence of Emi2 antisense morpholinos. After 45 min, Mos protein, Emi2 protein, or both were added. Samples were taken at indicated times, and pMAPK and cyclin B2 were examined by immunoblotting.