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. Author manuscript; available in PMC: 2007 Oct 17.
Published in final edited form as: Clin Lab Med. 2007 Sep;27(3):469–v. doi: 10.1016/j.cll.2007.05.002

Figure 6. Gating strategy.

Figure 6

The sequential gating strategy applied for the analysis of CD4+ and CD8+ T-cell responses is illustrated by the gates and arrows. Healthy donor PBMC were incubated with anti-CD3 and anti-CD28 antibodies in the presence of CD107aAx680 and monensin.

Note that CD3 molecules were visualized by intracellular labelling. After gating on single-cells, analysis was narrowed down on small lymphocytes. Live CD3+ T-cells were identified and further subdivided into CD4+ and CD8+ T-cells. These were subsequently analyzed for activation markers and cytokines of interest. Some pseudo-colour plots for possible antigen combinations are illustrated.