. Author manuscript; available in PMC: 2007 Oct 17.

Published in final edited form as: Clin Lab Med. 2007 Sep;27(3):469–v. doi: 10.1016/j.cll.2007.05.002

Table 1.

Detectors used in the VRC’s LSR II instruments and corresponding dyes

Detector	Conjugates	Non-conjugates
FSC	--
SSC	--
V450	Cascade Blue, Pacific Blue	DAPI^d, ViViD^d, CFP^pt
V545	QD545^O, Ax430, Pacific Orange	AquaBlue^d
V565	QD565^O
V585	QD585^O
V605	QD605^O
V655	QD655^O
V705	QD705^O
V800	QD800^O
B515	FITC⁺⁺⁺, Ax488	CFSE^c, GrViD^d, GFP^pt
B710	PerCP, PerCP-Cy5.5⁺⁺
G560	PE⁺⁺⁺, Ax532	CMTMR^c
G610	PE-TR/ECD⁺, TR, Ax594, PE-Ax594	EMA^d, OrViD^d, RFP^pt
G660	PE-Cy5⁺⁺, PE-Ax647	PI^d
G710	PE-Cy5.5⁺, PE-Ax700
G780	PE-Cy7⁺⁺, PE-Ax750
R660	APC⁺⁺, Ax633, Ax647
R710	APC-Cy5.5⁺, Ax660, Ax680, Ax700
R780	APC-Cy7⁺, APC-Ax750, Ax750

Detector nomenclature: laser light exciting the fluorochrome is indicated by the letter (V: violet, 407 nm; B: blue, 488 nm; G: green, 532 nm; R: red, 633 nm), the subsequent number specifying the peak photon emission wavelength measured by the band-pass filter used in front of the respective photomultiplier tubes (PMT).

⁺⁺⁺

many conjugates are commercially available,

⁺⁺

some conjugates are commercially available,

⁺

few conjugates are commercially available,

conjugates are made on a custom basis,

cellular dye,

dead cell marker dye,

^pt

protein tag.