Table 1.
Effect of ethanol on [3H]muscimol (3 nM) binding and diazepam-insensitive [3H]Ro 15–4513 (2 nM) binding in the membranes from the rat cerebellum and cerebral cortex.
Each value is mean ± S.E.M. of number of individual experiments indicated (n), each performed in triplicate.
| Group | % Inhibition of binding[3H]Muscimol (n) | [3H]Ro 15–4513 (n) |
|---|---|---|
| Cerebellum | ||
| Ethanol (1–50 mM) | < 5.0 (8) | < 5.0 (4) |
| Ethanol (100 mM) | 2.1 ± 1.6 (6) | 5.4 ± 2.3 (4) |
| Ethanol (200 mM) | 5.6 ± 1.1 (6) | 6.3 ± 2.1 (5) |
| Ethanol (500 mM) | 6.7 ± 0.9 (6) | 17.8 ± 0.8 (5) |
| Cerebral cortex | ||
| Ethanol (1–50 mM) | < 5.0 (7) | < 5.0 (5) |
| Ethanol (100 mM) | 5.4 ± 2.5 (6) | 7.9 ± 0.8 (5) |
| Ethanol (200 mM) | 6.6 ± 1.9 (7) | 10.4 ± 0.7 (5) |
| Ethanol (500 mM) | 10.1 ± 2.4 (7) | 21.9 ± 1.2 (5) |
Basal [3H]muscimol (3 nM) binding was found to be 1.38 ± 0.08 pmol/mg protein and 0.39 ± 0.05 pmol/mg protein in the membranes from the rat cerebellum and cerebral cortex, respectively. Basal [3H]Ro 15–4513 (2 nM) diazepam-insensitive binding was found to be 0.32 ± 0.02 pmol/mg protein and 0.027 ± 0.002 pmol/mg protein in the membranes from the rat cerebellum and cerebral cortex, respectively.