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. Author manuscript; available in PMC: 2008 Aug 24.
Published in final edited form as: Brain Res. 2007 Jul 26;1165:15–20. doi: 10.1016/j.brainres.2007.06.051

Table 2.

Effect of ethanol on [3H]muscimol (15 nM) binding to the immunoprecipitated δ-subunit-containing GABAA receptor assemblies in the rat cerebellum and hippocampus.

Each value is mean ± S.E.M. of three individual experiments, each performed in triplicate.

Treatment % Inhibition of binding
Cerebellum Hippocampus
Ethanol (5–50 mM) < 5.0 <5.0
Ethanol (200 mM) 7.8 ± 0.8 10.5 ± 1.7
Ethanol (500 mM) 12.4 ± 0.9 13.9 ± 2.1

[3H]muscimol (15 nM) binding to the solubilized GABAA receptors from the rat cerebellum and hippocampus was found to be 0.12 ± 0.01 pmol/mg protein (n=3) and 0.09 ± 0.01 pmol/mg protein (n=3), respectively.