Table 1.
Effect of prenatal/lactational atrazine exposure on spleen cell phenotype at 3 months of age
| Phenotypea | Malesb |
Femalesb |
||
|---|---|---|---|---|
| Unimmunized | Immunizedc | Unimmunized | Immunizedc | |
| CD4+: Atrazine | 1.7 ± 0.2 | 1.8 ± 0.1 | 2.2 ± 0.2 | 2.5 ± 0.3 |
| CD4+: Control | 1.8 ± 0.3 | 1.6 ± 0.2 | 1.9 ± 0.3 | 2.6 ± 0.3 |
| CD8+: Atrazine | 0.9 ± 0.1 | 0.8 ± 0.1 | 1.0 ± 0.1* | 1.2 ± 0.2 |
| CD8+: Control | 1.0 ± 0.1 | 0.8 ± 0.1 | 0.8 ± 0.1 | 1.3 ± 0.1 |
| B220+: Atrazine | 3.3 ± 0.3 | 3.8 ± 0.2 | 4.0 ± 0.3 | 4.6 ± 0.5 |
| B220+: Control | 3.8 ± 0.3 | 3.7 ± 0.5 | 3.6 ± 0.6 | 4.4 ± 0.4 |
| Totald: Atrazine | 6.8 ± 1.0 | 8.8 ± 1.6 | 11.6 ± 4.6 | 9.8 ± 1.9 |
| Totald: Control | 7.1 ± 1.1 | 8.5 ± 1.9 | 10.7 ± 3.0 | 9.7 ± 1.3 |
a
Spleen cells were stained with anti‐CD4 (T helper cell), anti‐CD8 (cytotoxic T cell) and anti‐B220 (B cell) and analyzed by flow cytometry.
b
All values are average number of cells of the indicated phenotype standardized to 107 cells and expressed with standard deviation. The data are representative of a single experiment the n of the atrazine and control groups were 14 and 16 animals respectively.
c
Spleen cells from animals immunized with HKSP.
d
Total splenocyte number includes monocytes and other cell types not enumerated through flow cytometry.
*
P < 0.05.