Figure 2.
Early expression of β-gal is essential for the therapy of established pulmonary metastases. BALB/c mice were inoculated intravenously with 0.5 ml of HBSS containing the following tumor cells: 105 CT26.CL25 (A–D) and 105 CT26.WT (E and F). Three days after tumor injection, they received a single i.v. injection of the different rVV shown in the figure. Where indicated (with IL-2, C–F), rIL-2 (100,000 Cetus units/mouse, twice a day) was administered i.p. starting 12 h after rVV injection and continued for 3 days. Mice were checked every day for survival. Data are the sum of two different, independent experiments in which at least five mice were included in each group.