Skip to main content
. Author manuscript; available in PMC: 2008 Sep 21.
Published in final edited form as: Mol Cell. 2007 Sep 21;27(6):951–961. doi: 10.1016/j.molcel.2007.08.019

Figure 5.

Figure 5

Ume6p is a substrate of APC/CCdc20 during G2-M stage of the cell cycle. Panel A. Haploid strain RSY1069 (T7-UME6, top panel) or RSY1070 (T7-UME6db1Δ) was treated with hydroxyurea (HU) for 2 hrs to arrest the cells in S phase. The cells were washed and placed in fresh medium and timepoints taken as indicated. Ume6p and Ume6pdb1Δ levels were determined by Western blot analysis for every timepoint. Tib1p served as a loading control. Panel B. The execution of anaphase was determined in the samples described in Panel A by monitoring the presence of separated nuclei by DAPI staining and fluorescent microscopy.