Figure 2. Ebony protein is localized exclusively to glia of the larval and adult brains.
(A-F) Ebony can be detected with a cytoplasmic distribution in cells of the wild-type (Canton-S) adult brain (A), the adult ventral nervous system (B), and the larval brain and ventral nervous systems (C), but is not specifically detected in the e1 mutant (E, F; note the lack of cell body staining in the mutant). Panel D shows co-localization of Ebony and Repo proteins in glial cells of the optic lobes. Repo can be detected in every Ebony-containing glial cell. Staining with the neuronal marker ELAV did not detect any Ebony-positive cells in brain tissues (data not shown).
