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. Author manuscript; available in PMC: 2008 Aug 2.
Published in final edited form as: Neuron. 2007 Aug 2;55(3):435–447. doi: 10.1016/j.neuron.2007.06.038

Figure 3. The Ebony protein oscillation can be detected by immunostaining in wild-type brains but is damped or eliminated in the tim01 mutant.

Figure 3

(A) Ebony immunofluorescence at two times of the cycle in wild-type brains. Brains were dissected at ZT2 and ZT21 from flies maintained in LD or at CT3.5 and CT21 in DD conditions. Ebony fluorescence was observed to be more intense during the day (or subjective day) than at night (Arrows indicate sites of prominent rhythmicity) (B) Ebony fluorescence intensity is weaker in tim01 than in the wild type during the day, and rhythmicity is damped in the mutant. (C) Average normalized fluorescence (pixel) intensity for different brain regions (optic lobes and protocerebrum) in wild type and tim01. In all cases, Ebony fluorescence was normalized to Repo antibody staining intensity. * p<0.01, ** p<0.05 for differences in fluorescence intensity at two times of day in wild type (two-tailed student’s t-test). Differences were not significant for tim01.