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. Author manuscript; available in PMC: 2008 Aug 2.
Published in final edited form as: Neuron. 2007 Aug 2;55(3):435–447. doi: 10.1016/j.neuron.2007.06.038

Figure 5. Many Ebony-containing cells stain positive for PER or are close to PER-containing neurons.

Figure 5

(A-C) Double labeling for Ebony and PER shows that Ebony glia are adjacent to the sLNv, LNd, DN1, and DN3 neurons. (D, E) In the optic medulla, Ebony protein is localized to PER-containing glia (Red = PER, Green = Ebony); these two panels show different regions within the optic medulla. TIM protein was also co-localized with Ebony in many optic lobe glial cells (data not shown). (F-I) Localization of Ebony relative to PDF. (F) pdf-Gal4-driven expression of UAS-mCD8GFP in the adult brain. Ebony antibody was used to visualize the localization of Ebony protein relative to PDF-expressing cells and their projections. (G-H) Ebony-containing cells are in close proximity to PDF cell projections in the dorsal brain (G, arrow shows one Ebony cell) or in the medulla (H). (I) a high magnification picture of the region in panel H indicated by the dotted rectangle (Red = Ebony, Green= PDF).