Figure 7. Per/tim RNA and protein cycling are normal in the e1 mutant.
(A-B) Quantitative RT-PCR shows that per and tim RNAs cycle in abundance in head tissues of wild-type (C-S) and e1 mutant flies. Values for per or tim RNA abundance were normalized to rp49 values (see Methods). Each curve represents 5 independent measures of RNA abundance from two independent fly head preparations (two measures from one, three measures from the other). Error bars indicate standard error. (C-D) TIM protein oscillates in abundance in C-S and e1 heads. TIM protein amounts are normalized to MAPK values for all timepoints. Each curve in panel D represents 3 independent measures of protein abundance from two independent fly head extracts. Error bars indicate standard error.
