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. Author manuscript; available in PMC: 2007 Oct 17.
Published in final edited form as: Neuron. 2007 Jul 19;55(2):217–230. doi: 10.1016/j.neuron.2007.06.029

Figure 3. Impaired oligodendrocyte progenitor differentiation in the spinal cord of yy1 conditional mutants.

Figure 3

(A) Confocal image of lumbar spinal cord sections from controls (ctrl) and yy1 cko mice at postnatal day 2, 11 and 18, stained with antibodies specific for NG2 (green) to identify progenitors, CC1 (red) to identify oligodendrocytes and DAPI (blue) to counterstain nuclei. Optical sections (Z = 1.0 μm; X = 12 μm) of confocal epifluorescence images were sequentially acquired and LSM software was used to merge images. Examples of NG2+/DAPI+ and CC1+/DAPI+cells selected for counting are shown in the boxed areas (arrowheads) while their relative position is shown at low magnification (arrows). Scale bar=50μm, 10μm in inserts. (B) Quantification of the data shown in panel A. The values indicate mean ± SD of cell counts obtained in 3-4 mice of each genotype per time point. (C) Co-localization of progenitor markers PDGFRα (red) and NG2 (green) in the spinal cord of yy1 cko mice at p18. Scale bar=20μm