Figure 4. In vitro ablation of yy1 in oligodendrocyte progenitors prevents their differentiation into oligodendrocytes.

(A) Primary oligodendrocyte progenitors isolated from the cortex of neonatal yy1^flox/flox mice were transduced with adenoviral vectors expressing the recombinase Cre (CMV-Cre). Forty-eight hours later the cells were differentiated by mitogen withdrawal. Immunoreactivity for O4 (green) was assessed 3 days later, while immunoreactivity for MBP, GalC or PLP (green) was assessed 5 days after transduction. Note that the Cre⁺ yy1^flox/flox cells (red) progressed to the O4⁺ stage, but were unable to differentiate into myelin-expressing cells. Scale bar = 50μm. (B) Quantification of three distinct experiments performed in duplicate. * p<0.05, ***p<0.001.