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. Author manuscript; available in PMC: 2007 Oct 17.
Published in final edited form as: Cell Cycle. 2006 Jun 1;5(11):1234–1239. doi: 10.4161/cc.5.11.2815

Figure 1.

Figure 1

MyoGEF interacts with non-muscle myosin II-A. (A) Schematic diagram of GFP-tagged MyoGEF. The numbers represent the amino acids in MyoGEF. The Dbl-homology (DH) and pleckstrin homology (PH) domains are shown. (B) GFP-MyoGEF colocalizes with non-muscle myosin II-A at the cleavage furrow. HeLa cells were transfected with a plasmid encoding GFP-tagged MyoGEF (green). Twenty-four hours after transfection, the cells were fixed and stained with an antibody specific for non-muscle myosin II-A (red). Note that both GFP-MyoGEF and non-muscle myosin II-A localize to the cleavage furrow (arrows in a–c). Bar, 10 μm. (C) MyoGEF can bind to non-muscle myosin II. GST-myosin-ROD region (but not GST alone) can bring down ThioHis-MyoGEF (compare lane 2 with lane 3). ThioHis-MyoGEF protein is detected by immonublot analysis using a specific antibody for MyoGEF (lanes 1 and 3). (D) Coomassie blue staining gel of GST-myosin-ROD protein used in (C).